Find the right Gibco media formulation for DMEM, DMEM/F-12, MEM and RPMI-1640 media.

Cell Culture Media Best Practices

Here are a few simple tips & tricks to help ensure your cell culture media is maintained for optimal performance.

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If refrigerated, warm your cell culture media before use. Your cells would be cold at 2-8°C, and you would be too!

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Keep your cell culture media protected from light. Light exposure degrades the essential vitamins in media that your cells need to grow.

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Once you have supplemented your cell culture media with FBS, place the complete media into the refrigerator (2-8°C ) to maintain performance.

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Use your supplemented media within 2-4 weeks to reduce the chances of contamination and the impact of pH drift.

Resources

Cell Culture & Transfection Learning Center
Access cell culture and transfection educational resources for better experiment planning and execution.

Gibco Cell Culture Basics
The Gibco Cell Culture Basics handbook and videos can help your lab attain reproducible results.

Related products

Support

Cell Culture & Transfection Support Center
Find technical support recommendations for your cell culture and transfection workflows, including tips for experimental setup and in-depth troubleshooting help.

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